In this work we set to develop and to validate a new in vivo frameless orthotopic Diffuse Intrinsic Pontine Glioma (DIPG) model based in the implantation of a guide-screw system. It consisted of a guide-screw also called bolt. a Hamilton syringe with a 26-gauge needle and an insulin-like 15-gauge needle. The guide screw is 2. https://editsbloomchices.shop/product-category/sweater-vests/